製品の概要

  • 製品名Anti-SCAP antibody
    SCAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SCAP
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 450-500 of Human SCAP (NP_036367.2).

  • ポジティブ・コントロール
    • HeLa, Jurkat and 293T whole cell lysates
  • 特記事項Shelf Life: 1 year from date of receipt

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
  • 配列類似性Belongs to the WD repeat SCAP family.
    Contains 1 SSD (sterol-sensing) domain.
    Contains 7 WD repeats.
  • ドメインCholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.
  • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0199 antibody
    • SCAP antibody
    • SCAP_HUMAN antibody
    • SREBF chaperone antibody
    • SREBF chaperone protein antibody
    • SREBP cleavage activating protein antibody
    • SREBP cleavage-activating protein antibody
    • Sterol regulatory element binding protein cleavage-activating protein antibody
    • Sterol regulatory element-binding protein cleavage-activating protein antibody
    see all

Anti-SCAP antibody 画像

  • All lanes : Anti-SCAP antibody (ab125186) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 140 kDa


    Exposure time : 30 seconds
  • ab125186, at 6 µg/mg lysate, detecting SCAP in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125186 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 30 seconds.

Anti-SCAP antibody (ab125186) 使用論文

ab125186 has not yet been referenced specifically in any publications.

Product Wall

DISCOUNT CODE: ***
Expiration date: September 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab125186 using mouse or rat samplesand include this cod...

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