The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
SCAND2 contains a SCAN domain, an 80 amino acid residue sequence with a L-X(6)-L motif at its core. This core is flanked by A, E, L, M, H and C residues that are frequently found in alpha-helices. The function of SCAND2 has not been determined.