Anti-SCAI 抗体 [EPR4128] (ab124688)

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製品の概要

製品名Anti-SCAI antibody [EPR4128]
SCAI 一次抗体製品一覧
製品の詳細
Rabbit monoclonal [EPR4128] to SCAI
アプリケーション適用あり: IHC-P, ICC/IF, WB, Flow Cytmore details
種交差性
交差種: Mouse, Rat, Human
免疫原
Synthetic peptide, corresponding to amino acid residues in Human SCAI.
ポジティブ･コントロール
- Human thymus, thyroid, and 293T cell lysates; SH-SY5Y cells.
特記事項
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

製品の状態Liquid
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（K_D 値） K_D = 5.80 x 10 ^-12 M Learn more about K_D
バッファーpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
精製度Protein A purified
ポリ/モノモノクローナル
クローン名EPR4128
アイソタイプIgG
研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
IHC-P		1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/5. See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).
ICC/IF		1/400. For unpurified, use 1/50.
WB		1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa). For unpurified, use 1/200.
Flow Cyt		1/160. For unpurified, use 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

機能Tumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.
配列類似性Belongs to the SCAI family.
細胞内局在Membrane. Nucleus. Cytoplasm. Nuclear localization is required for inhibition of MKL1.
Target information above from: UniProt accession Q8N9R8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 286205 Human
- Entrez Gene: 320271 Mouse
- Entrez Gene: 690538 Rat
- SwissProt: Q8N9R8 Human
- SwissProt: Q8C8N2 Mouse
- Unigene: 59504 Human
- Unigene: 204836 Mouse
- Unigene: 206053 Rat
別名
- C9orf126 antibody
- FLJ11758 antibody
- FLJ36664 antibody
- MGC120525 antibody
- MGC120526 antibody
- MGC120528 antibody
- NET40 antibody
- Protein SCAI antibody
- SCAI antibody
- SCAI_HUMAN antibody
- Suppressor of cancer cell invasion antibody
- Suppressor of cancer cell invasion protein antibody
see all

Anti-SCAI antibody [EPR4128] 画像

Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size : 70 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size : 70 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)

Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Flow Cytometry - Anti-SCAI antibody [EPR4128] (ab124688)

Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)

Lane 1 : Human Thymus lysate
Lane 2 : Human Thyroid lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size : 70 kDa
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size : 70 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)

Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Flow Cytometry - Anti-SCAI antibody [EPR4128] (ab124688)

Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by Flow cytometry (red) compared to a nonspecific negative control antibody (green).
Other-Anti-SCAI antibody [EPR4128](ab124688)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Anti-SCAI antibody [EPR4128] (ab124688) 使用論文

ab124688 has not yet been referenced specifically in any publications.

ab124688 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"