製品の概要

  • 製品名Anti-Sca1 / Ly6A/E antibody [EPR3355]
    Sca1 / Ly6A/E 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to residues in Mouse Sca1/Ly6A/E.

  • ポジティブ・コントロール
    • Mouse kidney cell lysate; Mouse kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能T-cell activation.
    • 組織特異性Widely expressed.
    • 配列類似性Contains 1 UPAR/Ly6 domain.
    • 翻訳後修飾O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Anti-Sca1 / Ly6A/E antibody [EPR3355] 画像

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution + Mouse kidney cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.
    • Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

    Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) 使用論文

    This product has been referenced in:
    • Okamoto M  et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P ; Mouse . Read more (PubMed: 24278321) »
    • Basile LA  et al. HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primates. PLoS One 7:e30434 (2012). IHC-P ; Mouse . Read more (PubMed: 22383962) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us. I have heard back from the lab: For WB, denaturing and reducing conditions were used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"