Rabbit polyclonal to Sca1 / Ly6A/E
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Sca1/ Ly6A/E.
- This antibody gave a positive signal in Mouse thymus and kidney tissue lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).
Contains 1 UPAR/Ly6 domain.
O-glycosylated. Not N-glycosylated.
Information by UniProt
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
Western blot - Anti-Sca1 / Ly6A/E antibody (ab93843)
All lanes :
Anti-Sca1 / Ly6A/E antibody (ab93843) at 1 µg/mlLane 1 :
Thymus (Mouse) Tissue LysateLane 2 :
Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
14 kDaObserved band size:
13 kDa (why is the actual band size different from the predicted?
)Additional bands at:
31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.Exposure time:
The band observed at 13 kDa could potentially be a cleaved form of Sca1/Ly6A/E due to the presence of a 26 amino acid signal peptide.
has not yet been referenced specifically in any publications.
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