Anti-Ly-6A/E (Sca-1) 抗体 - Hematopoietic Stem Cell Marker (ab93843)
Key features and details
- Rabbit polyclonal to Ly-6A/E (Sca-1) - Hematopoietic Stem Cell Marker
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ly-6A/E (Sca-1) antibody - Hematopoietic Stem Cell Marker
Ly-6A/E (Sca-1) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ly-6A/E (Sca-1) - Hematopoietic Stem Cell Marker -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Sca1/ Ly6A/E aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab104951) -
ポジティブ・コントロール
- This antibody gave a positive signal in Mouse thymus and kidney tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa). |
ターゲット情報
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機能
T-cell activation. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 UPAR/Ly6 domain. -
翻訳後修飾
O-glycosylated. Not N-glycosylated.
Not phosphorylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
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別名
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
see all
画像
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All lanes : Anti-Ly-6A/E (Sca-1) antibody - Hematopoietic Stem Cell Marker (ab93843) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThe band observed at 13 kDa could potentially be a cleaved form of Ly6A/E (Sca1) due to the presence of a 26 amino acid signal peptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab93843 は論文での使用が確認できていません。