Anti-SC35 抗体 (ab116103)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SC35
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 170-219 (KSKSSSVSRS RSRSRSRSRS RSPPPVSKRE SKSRSRSKSP PKSPEEEGAV) of Human SC35 (NP_003007).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
  • 配列類似性
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾
    Extensively phosphorylated on serine residues in the RS domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 35 kDa antibody
    • arginine/serine-rich 2 antibody
    • PR264 antibody
    • Protein PR264 antibody
    • SC 35 antibody
    • SC-35 antibody
    • SC35 antibody
    • Serine/arginine-rich splicing factor 2 antibody
    • SFRS 2 antibody
    • SFRS2 antibody
    • SFRS2A antibody
    • Splicing component 35 kDa antibody
    • Splicing component antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 2 antibody
    • Splicing factor SC35 antibody
    • Splicing speckle antibody
    • Splicing speckles antibody
    • SR splicing factor 2 antibody
    • SRp30b antibody
    • SRSF2 antibody
    • SRSF2_HUMAN antibody
    see all

画像

  • Anti-SC35 antibody (ab116103) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 25 kDa

プロトコール

参考文献

ab116103 has not yet been referenced specifically in any publications.

レビューと Q&A

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Thank you for submitting an Abreview of ab116103. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested species and application (mouse, ICC/IF), we would like t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH/3T3 mouse embryonic fibroblast)
Specification
NIH/3T3 mouse embryonic fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - PBS with 0.5% Triton-X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

投稿 Jun 01 2012

DISCOUNT CODES:

ab115184: x


ab116103: y


Ablaufdatum jeweils: dd mm 2012

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper der IF zu testen, annehmen möchten. Wie besprochen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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