製品の概要

  • 製品名Anti-SATB2 antibody
    SATB2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SATB2
  • 特異性ab69995 does not cross-react with SATB1.
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic 16 amino acid peptide near the amino terminus of the human SATB2.

  • ポジティブ・コントロール
    • Mouse brain tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).

ターゲット情報

  • 機能Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • 組織特異性High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • 関連疾患Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • 配列類似性Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • 翻訳後修飾Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • 細胞内局在Nucleus matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Anti-SATB2 antibody 画像

  • Lane 1 : Anti-SATB2 antibody (ab69995) at 2 µg/ml
    Lane 2 : Anti-SATB2 antibody (ab69995) at 4 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab69995 at 5 µg/ml staining SATB2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of SATB2 in Human Brain cells using ab69995 at 20 ug/ml.

Anti-SATB2 antibody (ab69995) 使用論文

This product has been referenced in:
  • Schütz B  et al. Satb2-Independent Acquisition of the Cholinergic Sudomotor Phenotype in Rodents. Cell Mol Neurobiol N/A:N/A (2014). Rat . Read more (PubMed: 25239161) »
  • Zhang J  et al. Roles of SATB2 in osteogenic differentiation and bone regeneration. Tissue Eng Part A 17:1767-76 (2011). IHC-P ; Mouse . Read more (PubMed: 21385070) »

See all 2 Publications for this product

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