製品の概要

  • 製品名Anti-SATB1 antibody
    SATB1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SATB1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-ESDEENRQKTRPRT

    , corresponding to internal sequence amino acids 636-649 of Human SATB1 (NP_002962.1).

  • ポジティブ・コントロール
    • MOLT4 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 86, 110 kDa (predicted molecular weight: 86 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
  • 組織特異性Expressed predominantly in thymus.
  • 配列類似性Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • 翻訳後修飾Sumoylated. Sumoylation promotes cleavage by caspases.
    Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
    Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
  • 細胞内局在Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 2 isoforms produced by alternative splicing.
  • 別名
    • DNA binding protein SATB1 antibody
    • DNA-binding protein SATB1 antibody
    • SATB homeobox 1 antibody
    • SATB1 antibody
    • SATB1_HUMAN antibody
    • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
    • Special AT rich sequence binding protein 1 antibody
    • Special AT-rich sequence-binding protein 1 antibody
    see all

Anti-SATB1 antibody 画像

  • Anti-SATB1 antibody (ab106941) at 0.3 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hour
  • ab106941 (3.8µg/ml) staining of paraffin embedded Human Thymus shows select nuclear staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-SATB1 antibody (ab106941) 使用論文

ab106941 has not yet been referenced specifically in any publications.

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