The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Use at 2-5 µg/mg of lysate.
SASS6 (Spindle assembly abnormal protein 6 homolog) is required for centrosome duplication. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. Overexpression results in excess foci bearing centriolar markers.
Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; centrosome; centriole. Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis.
Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.