The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Use at 2-5 µg/mg of lysate.
SASS6 (Spindle assembly abnormal protein 6 homolog) is required for centrosome duplication. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. Overexpression results in excess foci bearing centriolar markers.
Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; centrosome; centriole. Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis.
Spindle assembly abnormal protein 6 homolog antibody
Western blot - SASS6 antibody (ab85993)
All lanes : Anti-SASS6 antibody (ab85993) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa Observed band size: 74 kDa
Exposure time: 3 minutes
Immunoprecipitation - SASS6 antibody (ab85993)
Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.