製品の概要

  • 製品名Anti-SART3 antibody
    SART3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SART3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide within residues: KDLRLVTNRA GKPKGLAYVE YENESQASQA VMKMDGMTIK ENIIKVAISN, corresponding to internal sequence amino acids 828-877 of Human SART3 (NP_055521).

  • ポジティブ・コントロール
    • Human fetal small intestine lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
  • 組織特異性Ubiquitously expressed.
  • 関連疾患Defects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
  • 配列類似性Contains 8 HAT repeats.
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DSAP1 antibody
    • hSART 3 antibody
    • hSART-3 antibody
    • P100 antibody
    • P110 antibody
    • p110(nrb) antibody
    • RP11 13G14 antibody
    • SART 3 antibody
    • SART-3 antibody
    • SART3 antibody
    • SART3_HUMAN antibody
    • Squamous cell carcinoma antigen recognized by T cells 3 antibody
    • Squamous cell carcinoma antigen recognized by T-cells 3 antibody
    • Tat interacting protein of 110 kDa antibody
    • Tat-interacting protein of 110 kDa antibody
    • Tip110 antibody
    see all

Anti-SART3 antibody 画像

  • Anti-SART3 antibody (ab90871) at 1 µg/ml + Human fetal small intestine lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 110 kDa

Anti-SART3 antibody (ab90871) 使用論文

ab90871 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Sep 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"