製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SART3
  • 由来種
    Goat
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    MSNADFAKLFLRK

    , corresponding to C terminal amino acids 951-963 of Human SART3

  • ポジティブ・コントロール
    • Lysates of cell lines Daudi, Jurkat and MOLT4.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 110 kDa. (predicted molecular weight: 110 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
    • 組織特異性
      Ubiquitously expressed.
    • 関連疾患
      Defects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
    • 配列類似性
      Contains 8 HAT repeats.
      Contains 2 RRM (RNA recognition motif) domains.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Cytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DSAP1 antibody
      • hSART 3 antibody
      • hSART-3 antibody
      • P100 antibody
      • P110 antibody
      • p110(nrb) antibody
      • RP11 13G14 antibody
      • SART 3 antibody
      • SART-3 antibody
      • SART3 antibody
      • SART3_HUMAN antibody
      • Squamous cell carcinoma antigen recognized by T cells 3 antibody
      • Squamous cell carcinoma antigen recognized by T-cells 3 antibody
      • Tat interacting protein of 110 kDa antibody
      • Tat-interacting protein of 110 kDa antibody
      • Tip110 antibody
      see all

    画像

    • Anti-SART3 antibody (ab36137) at 1 µg/ml + Daudi lysate (RIPA buffer, 30µg total protein per lane).

      Predicted band size: 110 kDa
      Observed band size: 150 kDa (why is the actual band size different from the predicted?)



      Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Approx 150kDa band observed in lysates of Human Tcell line Jurkat and Human Burkitt's Lymphoma cell line DAUDI (calculated MW of 111kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature (Yang et al, Cancer Res. 1999 Aug 15;59(16):4056-63; PMID: 10463607).

    プロトコール

    参考文献

    This product has been referenced in:
    • Long L  et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem 289:8916-30 (2014). WB . Read more (PubMed: 24526689) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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