The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 32 kDa.
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
The evelope protein is a component of the viral envelope that plays a central role in virus morphogenesis and assembly. It may be sufficient to form virus like particles.
Cell Membrane; single pass membrane protein.
E protein antibody
Envelope protein antibody
Envelope small membrane protein antibody
protein E antibody
protein sM antibody
sM protein antibody
Western blot - SARS E protein antibody (ab22055)
Lane 1 : Anti-SARS E antibody (ab22055) at 1 µg/ml (Recombinant fusion protein containing amino acids 59-70 ) Lane 2 : Anti-SARS E antibody (ab22055) at 1 µg/ml (Recombinant fusion partner without these amino acids.)