製品の概要

  • 製品名
    Sarcosine Assay Kit
  • サンプルの種類
    Cell culture supernatant, Milk, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプ
    Quantitative
  • 検出感度
    > 1 µM
  • 検出範囲
    1 µM - 10000 µM
  • 全工程の試験時間
    1h 00m
  • 製品の概要

    Abcam's Sarcosine Assay Kit provides an accurate, convenient measure of sarcosine in variety biological samples. In the assay, sarcosine is specifically oxidized to generate a product that converts a colorless probe to a product with intense red color (?max = 570 nm) and which is also highly fluorescent (Ex/Em = 538/587 nm). Sarcosine is therefore easily detected by either colorimetric or fluorometric methods with detection range 1-10000 µM.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Sarcosine, a natural amino acid, plays important roles as intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine, etc. Detection of sarcosine level has wide applications in research and development.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Sarcosine Assay Buffer WM 1 x 30ml
    Sarcosine Enzyme Mix (Lyophilized) Green 1 vial
    Sarcosine Probe (in DMSO, anhydrous) Red 1 vial
    Sarcosine Standard (10 µmol) (Lyophilized) Yellow 1 vial
  • 研究分野
  • 関連性
    Sarcosine (N-methylglycine) is an intermediate and byproduct in the synthesis and degradation of glycine. Sarcosine is metabolized to glycine by sarcosine dehydrogenase, while glycine-N-methyl transferase generates sarcosine from glycine. Sarcosine is a natural amino acid found in muscles and other body tissues and plays important role as an intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine.
  • 別名
    • N methylglycine

アプリケーション

Our Abpromise guarantee covers the use of ab65338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies
  • 追加情報
    FuncS: Use at an assay dependent dilution.
    Note: Urine samples do not work well with the assay due to sample interferences.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 画像

    • Sarcosine Standard Curve performed using ab65338.

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    参考文献

    This product has been referenced in:
    • Barfeld SJ  et al. c-Myc Antagonises the Transcriptional Activity of the Androgen Receptor in Prostate Cancer Affecting Key Gene Networks. EBioMedicine 18:83-93 (2017). Read more (PubMed: 28412251) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for your enquiry. We have contacted our source to request details of the composition of the assay buffer, enzyme mix and probe and I am sorry they have regrettably informed me that this is commercially sensitive information and they a...

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    Thank you for your enquiry. Please find below the answers to your questions. 1. Biospecimens like body fluids (plasma/ serum), tissues, and cell culture samples can be used. However, urine samples will give interference with the assay. For c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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