Anti-Sarcomeric Alpha Actinin 抗体 [EP2529Y] (ab68167)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]
Sarcomeric Alpha Actinin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2529Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68167の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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IP |
1/20 - 1/40.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
IP
1/20 - 1/40. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
組織特異性
Expressed in both skeletal and cardiac muscle. -
関連疾患
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
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別名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab68167 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes :
Lane 1 : HepG2 whole cell lysate at 10 µg
Lane 2 : ab68167 + HepG2 whole cell lysate at 10 µg
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate
Observed band size: 103 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat heart lysate
Lane 3 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa -
Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking and dilution buffer: 5% NFDM /TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (50)
ab68167 は 50 報の論文で使用されています。
- Weihl CC et al. Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure. Acta Neuropathol 145:127-143 (2023). PubMed: 36264506
- Cattaneo P et al. DOT1L regulates chamber-specific transcriptional networks during cardiogenesis and mediates postnatal cell cycle withdrawal. Nat Commun 13:7444 (2022). PubMed: 36460641
- Gu X et al. Uninterrupted CAG repeat drives striatum-selective transcriptionopathy and nuclear pathogenesis in human Huntingtin BAC mice. Neuron 110:1173-1192.e7 (2022). PubMed: 35114102
- Royer C et al. ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis. Nat Commun 13:941 (2022). PubMed: 35177595
- Faustino D et al. Novel integrated workflow allows production and in-depth quality assessment of multifactorial reprogrammed skeletal muscle cells from human stem cells. Cell Mol Life Sci 79:229 (2022). PubMed: 35396689