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Synthetic peptide corresponding to a region within C terminal amino acids 829-878 of Human Sarcomeric Alpha Actinin (EQVIASFRIL ASDKPYILAE ELRRELPPDQ AQYCIKRMPA YSGPGSVPGA)
Our Abpromise guarantee covers the use of ab62298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 103 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry of Human Muscle lysate tissue at an antibody concentration of 4-8µg/ml using anti-ACTN2 antibody (ab62298). The arrows indicate positively stained skeletal muscle cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"