Anti-Sarcomeric Alpha Actinin 抗体 (ab113086)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Sarcomeric Alpha Actinin antibody
Sarcomeric Alpha Actinin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sarcomeric Alpha Actinin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Monkey, Zebrafish -
免疫原
Recombinant Human Sarcomeric Alpha Actinin.
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ポジティブ・コントロール
- Human Heart and Skeletal Muscle tissues; A431 and H1299 whole cell lysates; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113086の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
組織特異性
Expressed in both skeletal and cardiac muscle. -
関連疾患
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Entrez Gene: 641575 Zebrafish
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
see all -
別名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
画像
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ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry.
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ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Skeletal muscle tissue by Immunohistochemistry.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab113086) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
7.5% SDS PAGE. -
ab112086 at 1/200 dilution staining Sarcomeric Alpha Actinin in Paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113086 は論文での使用が確認できていません。