Anti-SAR1B 抗体 [EPR10105(B)] (ab155278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10105(B)] to SAR1B
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SAR1B antibody [EPR10105(B)]
SAR1B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10105(B)] to SAR1B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- 293T, fetal liver, fetal brain and Human skeletal muscle lysates; Human gastric carcinoma and brain tissue; 293T cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10105(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | (2) |
1/50 - 1/100.
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. -
組織特異性
Expressed in many tissues including small intestine, liver, muscle and brain. -
関連疾患
Defects in SAR1B are the cause of chylomicron retention disease (CMRD) [MIM:246700]; also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. -
配列類似性
Belongs to the small GTPase superfamily. SAR1 family. -
細胞内局在
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region. - Information by UniProt
-
参照データベース
- Entrez Gene: 51128 Human
- Entrez Gene: 66397 Mouse
- Entrez Gene: 287276 Rat
- Omim: 607690 Human
- SwissProt: Q9Y6B6 Human
- SwissProt: Q9CQC9 Mouse
- SwissProt: Q5HZY2 Rat
- Unigene: 432984 Human
see all -
別名
- ANDD antibody
- CMRD antibody
- GTBPB antibody
see all
画像
-
All lanes : Anti-SAR1B antibody [EPR10105(B)] (ab155278) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : Fetal brain tissue lysate
Lane 4 : Human skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAR1B with ab155278 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling SAR1B with ab155278 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunofluorescence analysis of 293T cells labeling SAR1B with ab155278 at a 1/50 dilution.
-
Intracellular flow cytometric analysis of permeabilized 293T cells using ab155278 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab155278 は 4 報の論文で使用されています。
- Lu Y et al. Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells. Oncol Lett 20:186 (2020). PubMed: 32952655
- Li X et al. Inhibition of Sar1b, the Gene Implicated in Chylomicron Retention Disease, Impairs Migration and Morphogenesis of Developing Cortical Neurons. Neuroscience 449:228-240 (2020). PubMed: 33002559
- Frisbie CP et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin. Cells 8:N/A (2019). PubMed: 31847122
- Smagris E et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 291:10659-76 (2016). WB . PubMed: 27013658