Anti-SAR1 抗体 (ab125871)
Key features and details
- Rabbit polyclonal to SAR1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SAR1 antibody -
製品の詳細
Rabbit polyclonal to SAR1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish -
免疫原
Recombinant fragment, corresponding to a region within N terminal amino acids 6-180 of Human SAR1 (Q9NR31).
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ポジティブ・コントロール
- 293T and A431 whole cell lysates; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab125871 is purified by antigen-affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). -
配列類似性
Belongs to the small GTPase superfamily. SAR1 family. -
細胞内局在
Endoplasmic reticulum. Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 56681 Human
- Entrez Gene: 20224 Mouse
- Entrez Gene: 361842 Rat
- Omim: 607691 Human
- SwissProt: Q9NR31 Human
- SwissProt: P36536 Mouse
- Unigene: 499960 Human
- Unigene: 594910 Human
see all -
別名
- COPII associated small GTPase antibody
- COPII-associated small GTPase antibody
- GTP binding protein SAR1a antibody
see all
画像
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All lanes : Anti-SAR1 antibody (ab125871) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE -
ab125871, at 1/500, staining SAR1 in methanol-fixed A431 cells by Immunofluorescence. Lower image is co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab125871 は 3 報の論文で使用されています。
- Phuyal S et al. Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1. EMBO J 41:e110596 (2022). PubMed: 35938214
- McKnight KL et al. Stimulator of interferon genes (STING) is an essential proviral host factor for human rhinovirus species A and C. Proc Natl Acad Sci U S A 117:27598-27607 (2020). PubMed: 33060297
- Vanhoutte D et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB ; Mouse . PubMed: 27669143