製品の概要

  • 製品名
    Anti-SAPS3 antibody
  • 製品の詳細
    Rabbit polyclonal to SAPS3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide from a region between residue 743 and the C-terminus (residue 793) of human SAPS3,( NP_060782.2).

  • ポジティブ･コントロール
    • Whole cell lysate from Hela cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance.
  • 組織特異性
    Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes.
  • 配列類似性
    Belongs to the SAPS family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • C11orf23 antibody
      • Chromosome 11 open reading frame 23 antibody
      • KIAA1558 antibody
      • PP6R3 antibody
      • PP6R3_HUMAN antibody
      • PPP6R3 antibody
      • Protein phosphatase 6 regulatory subunit 3 antibody
      • SAP190 antibody
      • SAPL antibody
      • SAPLa antibody
      • SAPS domain family member 3 antibody
      • SAPS domain family, member 3 antibody
      • Serine/threonine-protein phosphatase 6 regulatory subunit 3 antibody
      • Sporulation induced transcript 4 associated protein SAPL antibody
      • Sporulation-induced transcript 4-associated protein SAPL antibody
      see all

    画像

    • Western blot - Anti-SAPS3 antibody (ab72034)
      Western blot - Anti-SAPS3 antibody (ab72034)
      All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Predicted band size: 98 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)

    • Immunoprecipitation - Anti-SAPS3 antibody (ab72034)
      Immunoprecipitation - Anti-SAPS3 antibody (ab72034)
      Immunoprecipitation/ Western Blot of SAPS3.
      Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
      Lane 2: Control IgG.

      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 3 seconds.

    参考文献

    This product has been referenced in:
    • Gallegos LL  et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). Read more (PubMed: 27255161) »

    See 1 Publication for this product

    ab72034 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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