Anti-SAPS3 抗体 (ab72034)
Key features and details
- Rabbit polyclonal to SAPS3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SAPS3 antibody -
製品の詳細
Rabbit polyclonal to SAPS3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla -
免疫原
Synthetic peptide from a region between residue 743 and the C-terminus (residue 793) of human SAPS3,( NP_060782.2).
-
ポジティブ・コントロール
- Whole cell lysate from Hela cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance. -
組織特異性
Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes. -
配列類似性
Belongs to the SAPS family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 55291 Human
- Omim: 610879 Human
- SwissProt: Q5H9R7 Human
- Unigene: 503022 Human
-
別名
- C11orf23 antibody
- Chromosome 11 open reading frame 23 antibody
- KIAA1558 antibody
see all
画像
-
All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 98 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunoprecipitation/ Western Blot of SAPS3.
Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab72034 は 1 報の論文で使用されています。
- Gallegos LL et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161