製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SAP30BP
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 90-140 of Human SAP30BP (NP_037392.1)

  • ポジティブ・コントロール
    • HeLa, 293T, NIH3T3 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 34 kDa.
IP Use a concentration of 2 - 5 µg/ml.

ターゲット情報

画像

  • All lanes : Anti-SAP30BP antibody (ab99925) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 34 kDa


    Exposure time : 30 seconds
  • ab99925 at 1 µg/ml staining SAP30BP in HeLa cell lysate immunoprecipitated using ab99925 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded, left lane) or control IgG (right lane). Exposure time: 10 seconds

参考文献

ab99925 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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