製品の概要

  • 製品名Anti-SAP155 antibody
    SAP155 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SAP155
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR, corresponding to a region within N terminal amino acids 1-50 of Human SAP155

  • ポジティブ・コントロール
    • fetal thymus cell lysate, human liver tissue, human skin tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • 配列類似性Belongs to the SF3B1 family.
    Contains 11 HEAT repeats.
  • 翻訳後修飾Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
  • 細胞内局在Nucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hsh155 antibody
    • OTTHUMP00000205700 antibody
    • OTTHUMP00000205702 antibody
    • OTTHUMP00000225001 antibody
    • OTTHUMP00000225002 antibody
    • Pre mRNA processing 10 antibody
    • Pre mRNA splicing factor SF3b, 155 kDa subunit antibody
    • Pre-mRNA splicing factor SF3b 155 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 155 kDa subunit antibody
    • PRP10 antibody
    • PRPF10 antibody
    • SAP 155 antibody
    • SAP155 antibody
    • sf3b1 antibody
    • SF3B1_HUMAN antibody
    • SF3b155 antibody
    • Spliceosome associated protein 155 antibody
    • Spliceosome-associated protein 155 antibody
    • Splicing factor 3B subunit 1 antibody
    • Splicing factor 3b, subunit 1, 155kDa antibody
    see all

Anti-SAP155 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SAP155 with ab66774 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling SAP155 with ab66774 at 16µg/ml. Arrows indicate positively labelled epidermal cells. Magnification: 400X.

  • Anti-SAP155 antibody (ab66774) at 1/1.25 dilution + fetal thymus cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 146 kDa
    Observed band size : 146 kDa
    Additional bands at : >168 kDa. We are unsure as to the identity of these extra bands.
    gel concentration: 8%
  • ICC/IF image of ab66774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66774, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SAP155 antibody (ab66774) 使用論文

ab66774 has not yet been referenced specifically in any publications.

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