製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SAP130
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide: VSKKLEDIRTRYAF, corresponding to C terminal amino acids 1204-1217 of Human SAP130.

  • ポジティブ・コントロール
    • HeLa or Raji Lysate.
  • 特記事項
    Principal Names – SF3B3; RSE1; SAP130; SF3B130; SF3b130; KIAA0017; splicing factor 3b, subunit 3, 130kDa; spliceosome-associated protein 130; splicing factor 3b, subunit 3, 130 kDa. Official Gene Symbol - SF3B3. GenBank Accession Number – NP_036558. LocusLink ID - 23450 (human); 101943 (mouse). Gene Ontology terms - pre-mRNA splicing factor activity; small nuclear ribonucleoprotein; mRNA processing; mRNA splicing; protein complex assembly; spliceosome complex.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 136 kDa).Can be blocked with Human SAP130 peptide (ab22952).

ターゲット情報

  • 機能
    Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
  • 組織特異性
    Expressed in various cancer cell ines.
  • 配列類似性
    Belongs to the SAP130 family.
  • ドメイン
    The N-terminus may interact with a transcriptional coactivator.
    The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
  • 翻訳後修飾
    Acetylated.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 130 kDa Sin3-associated polypeptide antibody
    • FLJ12761 antibody
    • Histone deacetylase complex subunit SAP130 antibody
    • KIAA0017 antibody
    • mSin3A associated protein 130 antibody
    • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
    • RSE1 antibody
    • SAP 130 antibody
    • Sap130 antibody
    • SF3b 130 antibody
    • SF3b130 antibody
    • SF3B3 antibody
    • Sin3-associated polypeptide p130 antibody
    • Sin3A associated protein 130kDa antibody
    • Sin3A associated protein antibody
    • SP130_HUMAN antibody
    • Spliceosome associated protein 130 antibody
    • Splicing factor 3b subunit 3 130kDa antibody
    • Splicing factor 3b subunit 3 antibody
    • STAF 130 antibody
    • STAF130 antibody
    see all

画像

  • Anti-SAP130 antibody (ab3608) at 0.1 µg/ml + HeLa cells, nuclear lysate at 30 µg

    Predicted band size : 136 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab3608 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3608, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Zhang Z  et al. Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the survival of motoneuron (SMN) pre-mRNA. J Biol Chem : (2011). WB ; Human . Read more (PubMed: 21220421) »
  • Yoshimoto R  et al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB ; Human . Read more (PubMed: 19103666) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount
50 µg
Specification
Mouse embryonic fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 25 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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