製品の概要

  • 製品名Anti-SAM68 antibody [EPR3232]
    SAM68 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3232] to SAM68
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region within Human SAM68.

  • ポジティブ・コントロール
    • Jurkat, HeLa, A431 and A673 cell lysates Paraffin-embedded human kidney tissue HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 機能Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
    Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
  • 組織特異性Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
  • 配列類似性Belongs to the KHDRBS family.
    Contains 1 KH domain.
  • 発生段階Isoform 3 is only expressed in growth-arrested cells.
  • ドメインThe KH domain is required for binding to RNA.
    The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions.
  • 翻訳後修飾Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
    Acetylated. Positively correlates with ability to bind RNA.
    Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
    Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Nucleus. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ34027 antibody
    • GAP associated tyrosine phosphoprotein p62 antibody
    • GAP-associated tyrosine phosphoprotein p62 antibody
    • KH domain containing RNA binding signal transduction associated 1 antibody
    • KH domain-containing antibody
    • KHDR1_HUMAN antibody
    • Khdrbs1 antibody
    • p21 Ras GTPase activating protein associated p62 antibody
    • p21 Ras GTPase-activating protein-associated p62 antibody
    • p62 antibody
    • p68 antibody
    • RNA-binding antibody
    • Sam68 antibody
    • signal transduction-associated protein 1 antibody
    • Src associated in mitosis 68 kDa protein antibody
    • Src-associated in mitosis 68 kDa protein antibody
    see all

Anti-SAM68 antibody [EPR3232] 画像

  • Overlay histogram showing HeLa cells stained with ab109197 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109197, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-SAM68 antibody [EPR3232] (ab109197) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : A673 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 48 kDa
  • ab109197, at 1/250, staining SAM68 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab109197, at 1/250, staining SAM68 in HeLa cells by Immunofluorescence.

Anti-SAM68 antibody [EPR3232] (ab109197) 使用論文

ab109197 has not yet been referenced specifically in any publications.

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