Anti-Salmonella typhimurium LPS 抗体 [1E6] (ab8274)

製品の概要

  • 製品名
    Anti-Salmonella typhimurium LPS antibody [1E6]
    Salmonella typhimurium LPS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1E6] to Salmonella typhimurium LPS
  • 特異性
    This antibody is directed against lipopolisaccharides of Salmonella typhimurium
  • アプリケーション
    適用あり: Flow Cyt, WB, Dot blot, ELISA, ICC/IF, IHC-Frmore details
  • 免疫原

    Lipopolysaccharides of Salmonella typhimurium

  • 特記事項


    This antibody is used for the detection of LPS of Salmonella typhimurium

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    This antibody is used for the detection of LPS of Salmonella typhimurium
  • ポリ/モノ
    モノクローナル
  • クローン名
    1E6
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 17442963ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Salmonellae are Gram-negative , facultatively anaerobic bacteria of the family Enterobacteriaceae, made up of nonspore-forming rods, usually motile with flagella. Salmonella enterica serovar Typhimurium is the among the most common Salmonella serovars causing Salmonellosis food poisoning. S. typhimurium has a less alarming public image than S.typhi (which causes typhoid), but is a bigger health problem worldwide. Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.
  • 別名
    • S. typhimurium antibody
    • Salmonella typhimurium antibody
    • SALMONELLA TYPHIMURIUM LPS FACTOR 4 antibody

画像

  • Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.

    See Abreview

  • All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution

    Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
    Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
    Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
    Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344

    Lysates/proteins at 100000000 cells per lane.

    Secondary
    AP conjugated goat anti-mouse polyclonal at 1/4000 dilution

    Image courtesy of Aaron White by Abreview.

    Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.

    See Abreview

参考文献

This product has been referenced in:
  • Liebl D  et al. SopB-Mediated Recruitment of SNX18 Facilitates Salmonella Typhimurium Internalization by the Host Cell. Front Cell Infect Microbiol 7:257 (2017). WB, IF, Electron Microscopy ; Human . Read more (PubMed: 28664153) »
  • Zhang K  et al. Age-dependent enterocyte invasion and microcolony formation by Salmonella. PLoS Pathog 10:e1004385 (2014). IHC-P . Read more (PubMed: 25210785) »

See all 4 Publications for this product

レビューと Q&A


Unfortunately, we do not carry the biotinylated forms of these 2 antibodies, but we do carry biotinylation kits (e.g. http://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html - other sizes exist as well) if you li...

Read More

We have 5 antibodies to Salmonella typhimurium:

ab69238 - was raised against the whole bacteria thus we do not know where the antibody would bind.

ab8274 and ab65922: both were raised against LPS of the bacterium which I suppose is lo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Salmonella Typhimurium Cell lysate - other (Whole cell polysaccharides)
Loading amount
1e+008 cells
Specification
Whole cell polysaccharides
Treatment
Proteinase K (0.5mg/mL) for 1 h at 65C
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Username

Aaron White

Verified customer

投稿 Jan 27 2012

Thank you for contacting Abcam.

I have no added both Salmonella Typhimurium and Salmonella Enteritidis to our list of species and so you should now be able to submit anAbreview for this antibody.

If there is anything else I can help...

Read More

DISCOUNT CODE: ****** Expiration date:****** I am very pleased to hear you would like to accept our offer and test ab8274 in western blot. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Salmonella paratyphi Tissue sections (Salmonella ultrathin cryosections)
Specification
Salmonella ultrathin cryosections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 May 29 2008

Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich imm...

Read More

Both monoclonal antibodies recognize heat-killed Salmonella typhimurium. We have used the monoclonal antibodies (Mab) in ELISA according to the following scheme: antigen coating - Mab - Anti-mouse-IgG (conjugated), not in sandwich ELISA. The antibo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録