SALL2 overexpression 293T lysate (whole cell) (ab94229)
製品の概要
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製品名
SALL2 overexpression 293T lysate (whole cell) -
特記事項
ab94229 is a 293T cell transfected lysate in which Human SALL2 has been transiently over-expressed using a pCMV-SALL2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
The region-specific homeotic gene sal (spalt) of Drosophila encodes a zinc finger protein of unusual but characteristic structure. These unique features were used to isolate sal-like genes from humans. Two sal-like transcription units SALL1 and SALL2, located on chromosomes 16q12.1 and 14q11.1-q12.1 respectively, have been isolated and characterized. SALL1 and SALL2 transcripts are expressed in a limited number of adult organs, including the brain. SALL2 is evenly expressed in different brain areas. Transcripts of both genes can be detected in fetal brain neurons. The arrangement of sal-like zinc finger domains and their high degree of sequence similarity suggest a novel and conserved subfamily of human zinc finger transcription factors that is closely related to the Drosophila gene product encoded by the gene sal.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94229の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94229 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-SALL2 antibody (ab55723) at 1/500 dilution
Lane 1 :SALL2 overexpression 293T lysate (whole cell) (ab94229)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94229 は論文での使用が確認できていません。