The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration. PubMed: 21613322
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).
Transcriptional repressor involved in organogenesis.
Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss. Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
Belongs to the sal C2H2-type zinc-finger protein family. Contains 9 C2H2-type zinc fingers.
In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SALL1 antibody (ab31526)Image from Di Giovanni V et al, Development. 2011 Jul;138(13):2717-27. Epub 2011 May 25, Fig 4. DOI 10.1242/dev.059030/-/DC1
ab31526 staining SALL1 in metanephric blastema cells, murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab31526 stained Mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31526 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.