製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SAE2 / UBA2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human SAE2/ UBA2.

    (Peptide available as ab109093.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human brain and Human heart tissue lysates aswell as the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco2.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).

ターゲット情報

  • 機能
    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • パスウェイ
    Protein modification; protein sumoylation.
  • 配列類似性
    Belongs to the ubiquitin-activating E1 family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anthracycline associated resistance ARX antibody
    • Anthracycline-associated resistance ARX antibody
    • ARX antibody
    • FLJ13058 antibody
    • HRIHFB2115 antibody
    • SAE 2 antibody
    • SAE2 antibody
    • SAE2_HUMAN antibody
    • SUMO 1 activating enzyme subunit 2 antibody
    • SUMO activating enzyme subunit 2 antibody
    • SUMO-activating enzyme subunit 2 antibody
    • UBA2 antibody
    • UBA2 ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin like 1 activating enzyme E1B antibody
    • Ubiquitin like modifier activating enzyme 2 antibody
    • Ubiquitin-like 1-activating enzyme E1B antibody
    • UBLE1B antibody
    see all

画像

  • All lanes : Anti-SAE2 / UBA2 antibody (ab95835) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : Human brain tissue lysate - total protein (ab29466)
    Lane 7 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 71 kDa


    Exposure time : 20 minutes

プロトコール

参考文献

ab95835 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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