製品の概要

  • 製品名Anti-SAE1 (phospho S185) antibody
    SAE1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SAE1 (phospho S185)
  • 特異性ab106096 shows minimal reactivity by ELISA against the non-phosphorylated form of the immunising peptide
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Chimpanzee
  • 免疫原

    A synthetic peptide corresponding to a region surrounding serine 185 of Human SAE1.

  • ポジティブ・コントロール
    • HeLa cells treated with phosphatase inhibitors.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
ELISA 1/5000 - 1/25000.

ターゲット情報

  • 機能The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • 組織特異性Expression level increases during S phase and drops in G2 phase (at protein level).
  • パスウェイProtein modification; protein sumoylation.
  • 配列類似性Belongs to the ubiquitin-activating E1 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator of SUMO1 antibody
    • AOS1 antibody
    • HSPC140 antibody
    • Sae1 antibody
    • SAE1_HUMAN antibody
    • Sentrin/SUMO activating protein AOS1 antibody
    • SUA1 antibody
    • SUMO 1 activating enzyme E1 N subunit antibody
    • SUMO 1 activating enzyme subunit 1 antibody
    • SUMO-activating enzyme subunit 1 antibody
    • Ubiquitin like protein SUMO1 activating enzyme antibody
    • Ubiquitin-like 1-activating enzyme E1A antibody
    • UBL E1A antibody
    • UBLE1A antibody
    see all

Anti-SAE1 (phospho S185) antibody 画像

  • All lanes : Anti-SAE1 (phospho S185) antibody (ab106096) at 1/1000 dilution

    Lane 1 : HeLa whole cell protein
    Lane 2 : HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated secondary
    Developed using the ECL technique

    Predicted band size : 38 kDa

Anti-SAE1 (phospho S185) antibody (ab106096) 使用論文

ab106096 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106096.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"