製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SAE1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    MKGNGIVECLGPK

    , corresponding to C terminal amino acids 3374-346 of Human SAE1 .

  • ポジティブ・コントロール
    • Jurkat lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5% Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Tested in peptide ELISA: antibody detection limit dilution 1/8000.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
    • 組織特異性
      Expression level increases during S phase and drops in G2 phase (at protein level).
    • パスウェイ
      Protein modification; protein sumoylation.
    • 配列類似性
      Belongs to the ubiquitin-activating E1 family.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activator of SUMO1 antibody
      • AOS1 antibody
      • HSPC140 antibody
      • Sae1 antibody
      • SAE1_HUMAN antibody
      • Sentrin/SUMO activating protein AOS1 antibody
      • SUA1 antibody
      • SUMO 1 activating enzyme E1 N subunit antibody
      • SUMO 1 activating enzyme subunit 1 antibody
      • SUMO-activating enzyme subunit 1 antibody
      • Ubiquitin like protein SUMO1 activating enzyme antibody
      • Ubiquitin-like 1-activating enzyme E1A antibody
      • UBL E1A antibody
      • UBLE1A antibody
      see all

    画像



    • Predicted band size : 40 kDa


      ab5645 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    プロトコール

    参考文献

    This product has been referenced in:
    • Jones MC  et al. Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone. Proc Natl Acad Sci U S A 103:16272-7 (2006). Read more (PubMed: 17053081) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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