製品の概要

  • 製品名Anti-Sacsin antibody
    Sacsin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sacsin
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Sacsin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins.
  • 組織特異性Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas.
  • 関連疾患Spastic ataxia Charlevoix-Saguenay type
  • 配列類似性Contains 1 HEPN domain.
    Contains 1 J domain.
    Contains 1 ubiquitin-like domain.
  • ドメインThe ubiquitin-like domain mediates interaction with the proteasome.
    The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity.
  • 細胞内局在Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DnaJ homolog subfamily C member 29 antibody
    • DNAJC29 antibody
    • SACS antibody
    • SACS_HUMAN antibody
    • Sacsin antibody
    • Spastic ataxia of Charlevoix-Saguenay antibody
    see all

Anti-Sacsin antibody 画像

  • IHC image of Sacsin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80195, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • IF image of ab80195 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-Sacsin antibody (ab80195) 使用論文

ab80195 has not yet been referenced specifically in any publications.

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