Application
Western blot
Loading amount
36 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Bone - Tibia)
Specification
Bone - Tibia
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 30 kDa
Exposure
1 minute(s) and 35 second(s)
Positive control
Samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Helder Fonseca
Verified customer
投稿 Sep 25 2014