Synthetic peptide based on the catalytic domain of human MBTPS1. Immunizing peptide available on request - please contact a member of staff to ask for availability.
Our Abpromise guarantee covers the use of ab59870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | Use at an assay dependent concentration. | |
WB | 1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. |
ab59870 staining S1P in Human HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% donkey serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50) for 3 hours at 22°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
ab59870 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"