製品の概要
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製品名Anti-S1P antibody
S1P 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to S1P
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由来種Rabbit
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特異性We have a range of domain specific antibodies for this target. For a full list please see all MBTPS1 antibodies. ab59870 recognizes all four forms of MBTPS1 (1052, 746, 607 and 552 amino acids in length).
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide based on the catalytic domain of human MBTPS1. Immunizing peptide available on request - please contact a member of staff to ask for availability.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab59870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. |
ターゲット情報
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関連性S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain.
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細胞内局在Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein.
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参照データベース
- Entrez Gene: 8720 Human
- Omim: 603355 Human
- SwissProt: Q14703 Human
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別名
- kexin isozyme 1 antibody
- KIAA0091 antibody
- MBTPS1 antibody
see all
画像
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Western blot - Anti-S1P antibody (ab59870)Anti-S1P antibody (ab59870) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Exposure time: 16 minutes
The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates. -
Immunocytochemistry/ Immunofluorescence - Anti-S1P antibody (ab59870)This image is courtesy of an Abreview submitted by Armen Petrosyanab59870 staining S1P in Human HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% donkey serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50) for 3 hours at 22°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
プロトコール
参考文献
ab59870 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"