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RabMAb

Anti-S100A9 抗体 [EPR3556] (ab92468)

製品の概要

  • 製品名
    Anti-S100A9 antibody [EPR3556]
    S100A9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3556] to S100A9
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of Human S100A9 (UniProt ID: P06702).

  • ポジティブ・コントロール
    • WB: Human tonsil lysate and peripheral blood leukocyte cell lysate IHC-P: Human spleen tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/1000 - 1/10,000. Predicted molecular weight: 13 kDa.

    Is unsuitable for ICC/IF or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • 組織特異性
      Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • 配列類似性
      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • 翻訳後修飾
      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • 細胞内局在
      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Anti-S100A9 antibody [EPR3556] 画像

    • All lanes : Anti-S100A9 antibody [EPR3556] (ab92468) at 1/1000 dilution

      Lane 1 : Human tonsil cell lysate
      Lane 2 : Human peripheral blood leukocyte cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 13 kDa
    • ab92468, at a 1/100 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.

    Anti-S100A9 antibody [EPR3556] (ab92468) 使用論文

    This product has been referenced in:
    • Wei J  et al. Perinatal exposure to bisphenol A exacerbates nonalcoholic steatohepatitis-like phenotype in male rat offspring fed on a high-fat diet. J Endocrinol 222:313-25 (2014). Read more (PubMed: 25112833) »
    • Junker H  et al. Stage-Related Alterations in Renal Cell Carcinoma - Comprehensive Quantitative Analysis by 2D-DIGE and Protein Network Analysis. PLoS One 6:e21867 (2011). WB, IHC-P ; Human . Read more (PubMed: 21760917) »

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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