Anti-S100A9 抗体 [CF-557] (Phycoerythrin) (ab112210)

製品の概要

  • 製品名
    Anti-S100A9 antibody [CF-557] (Phycoerythrin)
    S100A9 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CF-557] to S100A9 (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified granulocyte antigen of Human origin.

  • ポジティブ・コントロール
    • Human peripheral blood leukocytes
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • 組織特異性
    Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • 配列類似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 翻訳後修飾
    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • 細胞内局在
    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Leukocyte L1 complex heavy chain antibody
    • 60B8AG antibody
    • CAGB antibody
    • Calgranulin B antibody
    • Calgranulin-B antibody
    • Calprotectin L1H subunit antibody
    • CFAG antibody
    • CGLB antibody
    • Cystic fibrosis antigen B antibody
    • L1AG antibody
    • Leukocyte L1 complex heavy chain antibody
    • LIAG antibody
    • MAC387 antibody
    • MIF antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor-related protein 14 antibody
    • MRP 14 antibody
    • MRP-14 antibody
    • MRP14 antibody
    • Myeloid-related protein 14 antibody
    • NIF antibody
    • OTTHUMP00000015331 antibody
    • p14 antibody
    • Protein S100-A9 antibody
    • S100 A9 antibody
    • S100 calcium binding protein A9 antibody
    • S100 calcium binding protein A9 calgranulin B antibody
    • S100 calcium-binding protein A9 antibody
    • S100A9 antibody
    • S10A9_HUMAN antibody
    see all

Anti-S100A9 antibody [CF-557] (Phycoerythrin) 画像

  • Tissue Distribution by Flow Cytometry Analysis:
    Human peripheral blood leukocytes labeled with ab112210 at a concentration of 2.0 µg for 106 cells, and PE secondary antibody.
    Percentage of cells stained above control: 74.7%
    Isotypic Control (shaded): Purified Mouse IgG1

プロトコール

Anti-S100A9 antibody [CF-557] (Phycoerythrin) (ab112210) 使用論文

ab112210 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
Yes - BD Kit
Gating Strategy
Live cells
Specification
PBMC
Fixation
BD Kit
Username

Abcam user community

Verified customer

投稿 May 19 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録