Anti-S100A4 抗体 [1F12-1G7] (ab93283)
Key features and details
- Mouse monoclonal [1F12-1G7] to S100A4
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-S100A4 antibody [1F12-1G7]
S100A4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1F12-1G7] to S100A4 -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.
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ポジティブ・コントロール
- HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.
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特記事項
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
1F12-1G7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93283の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 6275 Human
- Entrez Gene: 20198 Mouse
- Omim: 114210 Human
- SwissProt: P26447 Human
- SwissProt: P07091 Mouse
- Unigene: 654444 Human
- Unigene: 3925 Mouse
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別名
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
画像
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 37.22 kDa why is the actual band size different from the predicted?Predicted band size for the immunogen in 37.22 kDa.
This image was generated using the ascites version of the product.
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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All lanes : Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)
Lane 1 : cell lysate from S100A4 transfected 293T cell line
Lane 2 : cell lysate from non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 11.7 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
This image was generated using the ascites version of the product.
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Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab93283 は 7 報の論文で使用されています。
- Jia M et al. Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis. Front Physiol 13:778479 (2022). PubMed: 35309056
- Gundlach JP et al. Paracrine Interaction of Cholangiocellular Carcinoma with Cancer-Associated Fibroblasts and Schwann Cells Impact Cell Migration. J Clin Med 11:N/A (2022). PubMed: 35628911
- Ugai K et al. Inhibition of the SET8 Pathway Ameliorates Lung Fibrosis Even Through Fibroblast Dedifferentiation. Front Mol Biosci 7:192 (2020). PubMed: 32850975
- Méndez-Barbero N et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500
- Nguy?n-Thanh T et al. Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice. Int J Mol Med 41:95-106 (2018). PubMed: 29115561
- Zhang W et al. S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis. Front Immunol 9:1216 (2018). PubMed: 29910813
- Wilding C et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). PubMed: 24595072