Goat polyclonal to S100A4
Both variants (NP_002952.1; NP_062427.1) represent identical protein.
Mouse, Rat, Dog
, corresponding to C terminal amino acids 86-98 of Human S100A4
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
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Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Information by UniProt
calcium Placental protein antibody
Western blot - S100A4 antibody (ab58597)
Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg
Predicted band size :
Observed band size :
13 kDa (
why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (ab58597)
ab58597 at a concentration of 2µg/ml staining S100A4 in l
ymphatic cells in the lamina propria of human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Antigen retrieval was with a citrate buffer.
This product has been referenced in:
et al. Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target. Mol Cell Proteomics 12:3778-92 (2013).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"