製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to S100A4
  • 特異性
    Both variants (NP_002952.1; NP_062427.1) represent identical protein.
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    CNEFFEGFPDKQP

    , corresponding to C terminal amino acids 86-98 of Human S100A4

  • ポジティブ・コントロール
    • Human Placenta lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).

ターゲット情報

  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

画像

  • Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 12 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab58597 at a concentration of 2µg/ml staining S100A4 in lymphatic cells in the lamina propria of human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Antigen retrieval was with a citrate buffer.

     

参考文献

This product has been referenced in:
  • Rasanen K  et al. Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target. Mol Cell Proteomics 12:3778-92 (2013). Read more (PubMed: 24037664) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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