製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to S100A4
  • アプリケーション
    適用あり: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human S100A4.

    (Peptide available as ab41531.)

  • ポジティブ・コントロール
    • This antibody gives a positive signal in NIH3T3 whole cell lysate and when tested against Human S100A4 Recombinant Protein. This antibody gives a positive signal in the following formaldehyde fixed cell lines: MCF-7.
  • 特記事項

    Blocking peptide available ab41531.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/250. Predicted molecular weight: 12 kDa.Can be blocked with Human S100A4 peptide (ab41531).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Anti-S100A4 antibody 画像

  • ab41532 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ICC/IF image of ab41532 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41532 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-S100A4 antibody (ab41532) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-S100A4 antibody (ab41532) at 1/250 dilution + Recombinant human S100A4 protein (ab83650) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa


    Exposure time : 20 minutes

    The S100A4 tagged recombinant protein has a molecular weight of 36.8 kDa.

Anti-S100A4 antibody (ab41532) 使用論文

This product has been referenced in:
  • Kim JY  et al. Apamin suppresses biliary fibrosis and activation of hepatic stellate cells. Int J Mol Med 39:1188-1194 (2017). IHC-P ; Mouse . Read more (PubMed: 28405682) »
  • Taniguchi T  et al. Fibrosis, vascular activation, and immune abnormalities resembling systemic sclerosis in bleomycin-treated Fli-1-haploinsufficient mice. Arthritis Rheumatol 67:517-26 (2015). IHC ; Mouse . Read more (PubMed: 25385187) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human umbilical vein endothelial cell)
Specification
human umbilical vein endothelial cell
Fixative
Cytofix Fixation and Permeabilization
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human dermal microvascular endothelial cell)
Loading amount
30 µg
Specification
Human dermal microvascular endothelial cell
Treatment
30mM Glucose, 10nG TNF-alpha for 24hrs, 48hrs
Gel Running Conditions
Reduced Denaturing (4-20% gradient SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2013

ab83650, S100A4 protein is actually a GST-tagged protein which has a total MW of 36.8 kDa. GST(glutathione S-transferase) which is commonly applied in recombinant protein is about 26kDa. Therefore, S100A4 protein (12kDa) + GST(26kDa) is around 38kDa, a...

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Thank you for your reply.

As agreed I added one unit of ab41532, free of charge, to the order number ***** in compensation of the problem experienced with ab41532 ordered in January 2012.

Please remind your customers that our Abpromis...

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Reply to CCE ******* and CCE ******** as I assume both complaints are from the same customer.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear both antibodies ab41532 and ab106777 are not provi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cryosection-Heart)
Specification
Cryosection-Heart
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 02 2012

According to our records, ab41532 was proving difficult to use and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the d...

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DISCOUNT CODE: ***********
Expiration date: ************

I am very pleased to hear you would like to accept our offer and test ab41532 in sheep tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redee...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with ab41532. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Jan 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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