製品の概要

  • 製品名Anti-S100A3 antibody
    S100A3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to S100A3
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF, corresponding to N terminal amino acids 1-50 of Human S100A3

  • ポジティブ・コントロール
    • Human skin tissue or OVCAR3 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

Anti-S100A3 antibody 画像

  • Anti-S100A3 antibody (ab62277) at 5 µg/ml + OVCAR3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000

    Predicted band size : 12 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 59 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling S100A3 with ab62277 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.

Anti-S100A3 antibody (ab62277) 使用論文

ab62277 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62277.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"