製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to S100A3
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF, corresponding to N terminal amino acids 1-50 of Human S100A3

  • ポジティブ・コントロール
    • Human skin tissue or OVCAR3 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

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  • Anti-S100A3 antibody (ab62277) at 5 µg/ml + OVCAR3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000

    Predicted band size : 12 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 59 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling S100A3 with ab62277 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.

参考文献

ab62277 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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