Anti-S100 beta 抗体 - Astrocyte Marker (ab41548)

製品の概要

  • 製品名Anti-S100 beta antibody - Astrocyte Marker
    S100 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to S100 beta - Astrocyte Marker
  • アプリケーション適用あり: IHC-P, WB, IHC-FoFrmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Pig
    交差が予測される動物種: Rabbit, Cow, Human, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Rat S100 beta.

    (Peptide available as ab41547.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates; Brain (Rat), Spinal Cord (Mouse), Spinal Cord (Rat)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).Can be blocked with Rat S100 beta peptide (ab41547).
IHC-FoFr 1/3000.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
    • 組織特異性Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
    • 配列類似性Belongs to the S-101 family.
      Contains 2 EF-hand domains.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NEF antibody
      • Protein S100 B antibody
      • Protein S100-B antibody
      • S 100 calcium binding protein beta chain antibody
      • S 100 protein beta chain antibody
      • S-100 protein beta chain antibody
      • S-100 protein subunit beta antibody
      • S100 antibody
      • S100 calcium binding protein beta (neural) antibody
      • S100 calcium-binding protein B antibody
      • S100 protein beta chain antibody
      • S100B antibody
      • S100B_HUMAN antibody
      • S100beta antibody
      see all

    Anti-S100 beta antibody - Astrocyte Marker 画像

    • ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

      At this dilution factor, there is very good delineation of astrocyte processes.

      See Abreview

    • All lanes : Anti-S100 beta antibody - Astrocyte Marker (ab41548) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Spinal Cord (Mouse) Tissue Lysate
      Lane 4 : Spinal Cord (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 11 kDa
      Observed band size : 9 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
    • ab41548 staining S100 beta in Zebrafish body, showing T/S of spinal cord region by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • ab41548 staining S100 beta in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

      See Abreview

    Anti-S100 beta antibody - Astrocyte Marker (ab41548) 使用論文

    This product has been referenced in:
    • Chiu PS & Lai SC Matrix metalloproteinase-9 leads to blood-brain barrier leakage in mice with eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis. Acta Trop 140C:141-150 (2014). Mouse . Read more (PubMed: 25158284) »
    • Weissmann L  et al. IKKe is key to induction of insulin resistance in the hypothalamus, and its inhibition reverses obesity. Diabetes 63:3334-45 (2014). Read more (PubMed: 24812431) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20min
    Permeabilization No
    Specification brain
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Sep 29 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (cerebellum)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20min
    Permeabilization No
    Specification cerebellum
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Aug 27 2015

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Rat Tissue sections (Amygdala brain slices)
    Specification Amygdala brain slices
    Permeabilization Yes - Triton X
    Fixative Paraformaldehyde
    Username

    Jason Teem

    Verified customer

    投稿 Sep 17 2014

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
    Sample Human Serum (serum)
    Specification serum
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Aug 10 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Human Tissue sections (Colon)
    Specification Colon
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Aug 07 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Goat Tissue sections (Small intestine)
    Specification Small intestine
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Aug 01 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Cat Tissue sections (Small intestine)
    Specification Small intestine
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jul 31 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Zebrafish Tissue sections (T/S body)
    Specification T/S body
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jul 31 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Marmoset (common) Tissue sections (Samll intestine)
    Specification Samll intestine
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jul 31 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Quail Tissue sections (Embryo)
    Specification Embryo
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jul 28 2014

    1-10 of 17 Abreviews or Q&A

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