This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/50 - 1/100.
1/50 - 1/100.
Use a concentration of 10 - 20 µg/ml.
Incubate for 2 hours in the dark at RT. The incubation can also be left overnight at 4ºC.
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Belongs to the S-100 family. Contains 2 EF-hand domains.
Overlay histogram showing SH-SY5Y cells stained with ab76749 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76749, 0.1μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Zhu X et al. Schwann cell proliferation and differentiation that is induced by ferulic acid through MEK1/ERK1/2 signalling promotes peripheral nerve remyelination following crush injury in rats. Exp Ther Med12:1915-1921 (2016).
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