Anti-S100 抗体 [B32.1] (ab7852)
Key features and details
- Mouse monoclonal [B32.1] to S100
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-S100 antibody [B32.1]
S100 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B32.1] to S100 -
由来種
Mouse -
特異性
This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD). -
アプリケーション
適用あり: IHC-FoFrmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Cow S100.
Database link: P02639 -
ポジティブ・コントロール
- Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
B32.1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7852の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-FoFr |
1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.
|
特記事項 |
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IHC-FoFr
1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4. |
ターゲット情報
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機能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
組織特異性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
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別名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
画像
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ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30
µ m coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab7852 は 17 報の論文で使用されています。
- Galiakberova AA et al. IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Neurochem Res 47:952-966 (2022). PubMed: 34855047
- Gomez-Sintes R et al. Targeting retinoic acid receptor alpha-corepressor interaction activates chaperone-mediated autophagy and protects against retinal degeneration. Nat Commun 13:4220 (2022). PubMed: 35864098
- Arotcarena ML et al. Pilot Study Assessing the Impact of Intrathecal Administration of Variants AAV-PHP.B and AAV-PHP.eB on Brain Transduction in Adult Rhesus Macaques. Front Bioeng Biotechnol 9:762209 (2021). PubMed: 34869273
- Alhindi A et al. Terminal Schwann cells at the human neuromuscular junction. Brain Commun 3:fcab081 (2021). PubMed: 33977269
- Wu P et al. Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair. Bioact Mater 6:3515-3527 (2021). PubMed: 33842738