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Our Abpromise guarantee covers the use of ab66041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21040789|
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 11 kDa.
This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100.
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|IHC-P||1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling S100 with ab66041 at a dilution of 1/300 for 10 minutes at room temperature. Antigen retrieval was performed by boiling tissue sections in 10mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"