製品の概要

  • 製品名Anti-S100 antibody [6G1]
    S100 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6G1] to S100
  • 特異性Clone 6G1 is specific for S-100 beta beta and and S100 alpha beta
  • アプリケーション適用あり: Other, Flow Cyt, ELISA, Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human S-100 protein

  • 特記事項Concentration varies from lot to lot and can be provided on request.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Other Use at an assay dependent concentration.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.

ターゲット情報

  • 機能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 組織特異性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 配列類似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [6G1] 画像

  • ab85137 staining S100 in the Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. ab96879, Goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

Anti-S100 antibody [6G1] (ab85137) 使用論文

ab85137 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - saponine
Sample Human Cell (melanoma cell line)
Specification melanoma cell line
Gating Strategy cell line only
Preparation Cell harvesting/tissue preparation method: EDTA (Versen) for harvesting cells
Sample buffer: PBS
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投稿 Jul 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"