製品の概要

  • 製品名Anti-S100 antibody [4B3]
    S100 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4B3] to S100
  • 特異性Specific for alpha-beta heterodimer and beta-beta homodimer.
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) (Human brain).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
IHC-P 1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See Abreview.
IHC-Fr 1/100.
ICC 1/500.

ターゲット情報

  • 機能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 組織特異性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 配列類似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [4B3] 画像

  • ab14849 staining S100 in Human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview



  • Predicted band size : 21 kDa

    Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD, Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD,

  • ab14849 at 1/3000 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody. A biotinylated goat anti-mouse IgG was used as the secondary.

    See Abreview

  • ab14849 staining S100 in the Human Melanoma cell line 1205Lu xenograph tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X100 and blocked with a commercial block solution for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris EDTA at pH 9. The sample was incubated with primary antibody (1/300 in blocking solution) at 4°C for 18 hours. A Biotin-conjugated goat polyclonal to mouse IgG (1/50) was used as secondary antibody.

    See Abreview

Anti-S100 antibody [4B3] (ab14849) 使用論文

This product has been referenced in:
  • Lopez-Rivera E  et al. Inducible nitric oxide synthase drives mTOR pathway activation and proliferation of human melanoma by reversible nitrosylation of TSC2. Cancer Res 74:1067-78 (2014). IHC ; Human . Read more (PubMed: 24398473) »
  • Sun Y  et al. The expression and significance of neuronal iconic proteins in podocytes. PLoS One 9:e93999 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24699703) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Blocking step from Novolink Kit as blocking agent for 30 minute(s) · Concentration: 0.4µg/mL · Temperature: 21°C
Sample Human Cultured Cells (Melanoma)
Specification Melanoma
Permeabilization Yes - 0.2% Triton-X, 15 min
Fixative Paraformaldehyde
Username

Stacey Dmello

Verified customer

投稿 Jul 23 2013

Thank you for contacting us and your patience.

We recommend to use purified antibody in a concentration og 1ug/ml for WB. This recommended concentration needs to be optimised for each sample and experiment by the user but it is a good approxim...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (C57B/J mouse Vagus Nerve)
Specification C57B/J mouse Vagus Nerve
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk McManus

Verified customer

投稿 Mar 06 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Marmoset colon)
Specification Marmoset colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21C°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Melanoma cell line 1205Lu xenopraph)
Specification Melanoma cell line 1205Lu xenopraph
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization Yes - 0.1% triton x-100
Blocking step Commercially available block solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Hongwei Shao

Verified customer

投稿 Mar 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (rat brain)
Specification rat brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: No buffer: citric acid pH6
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"