Anti-S100 alpha 抗体 [EPR5251] (ab109384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5251] to S100 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-S100 alpha antibody [EPR5251]
S100 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5251] to S100 alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 5.00 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5251 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109384の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
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ICC/IF |
1/100 - 1/250.
|
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
組織特異性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
- SwissProt: P35467 Rat
- Unigene: 515715 Human
see all -
別名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
画像
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Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution + C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : C2C12 cell lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Human brain lysate
Lane 5 : HT-1080 lysate
Lane 6 : Mouse heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab109384 は論文での使用が確認できていません。