The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Use at an assay dependent concentration.
機能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
組織特異性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
配列類似性Belongs to the S-100 family. Contains 2 EF-hand domains.