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RabMAb

Anti-S100 alpha 2 抗体 [EPR5392] (ab109494)

製品の概要

  • 製品名
    Anti-S100 alpha 2 antibody [EPR5392]
    S100 alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5392] to S100 alpha 2
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues at the C-terminus of Human S100 alpha 2.

  • ポジティブ・コントロール
    • HACAT, BxPC3, and A431 cell lysates; Human skin and Human squamous cell cervical carcinoma tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-S100 alpha 2 antibody (Alexa Fluor® 488) [EPR5392] (ab205456)

    Anti-S100 alpha 2 antibody [EPR5392] (HRP) (ab205754)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
  • 組織特異性
    A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes.
  • 配列類似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 発生段階
    Preferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAN19 antibody
    • MGC111539 antibody
    • Protein S 100L antibody
    • Protein S-100L antibody
    • Protein S100 A2 antibody
    • Protein S100-A2 antibody
    • Protein S100L antibody
    • S100 A2 antibody
    • S100 calcium binding protein A2 antibody
    • S100 calcium-binding protein A2 antibody
    • S100A2 antibody
    • S100L antibody
    • S10A2_HUMAN antibody
    see all

画像

  • Overlay histogram showing A431 cells stained with ab109494 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109494, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-S100 alpha 2 antibody [EPR5392] (ab109494) at 1/1000 dilution

    Lane 1 : HACAT cell lysate
    Lane 2 : BxPC3 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 11 kDa
  • ab109494 at 1/250 dilution staining S100 alpha 2 in paraffin-embedded Human squamous cell cervical carcinoma tissue
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using ab109494 at 1/250 dilution.

参考文献

This product has been referenced in:
  • Kumar M  et al. Prognostic significance of cytoplasmic S100A2 overexpression in oral cancer patients. J Transl Med 13:8 (2015). IHC-P ; Human . Read more (PubMed: 25591983) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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