製品の概要

  • 製品名
    Anti-S tag antibody [SBSTAGa]
    S tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SBSTAGa] to S tag
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, Flow Cyt, ELISA, Purificationmore details
  • 免疫原

    Recombinant full length protein (see pET-32a-c(+) vector from Novagen)

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    SBSTAGa
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
Purification Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.
  • 別名
    • KETAAAKFERQHMDS tag antibody antibody
    • S peptide antibody
    • S peptide epitope tag antibody

Anti-S tag antibody [SBSTAGa] 画像

  • ab24838 staining human HeLa cells by ICC/IF.  Cells were PFA fixed and blocked with 3% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C.  A Cy2® conjugated donkey anti-mouse antibody, diluted 1/300 was used as the secondary. 

    The slide was stained with Dapi, S Tag-Cy2 and CREST-Cy3.

    See Abreview

Anti-S tag antibody [SBSTAGa] (ab24838) 使用論文

This product has been referenced in:
  • Makhmoudova A  et al. Identification of multiple phosphorylation sites on maize endosperm starch branching enzyme IIb, a key enzyme in amylopectin biosynthesis. J Biol Chem 289:9233-46 (2014). WB . Read more (PubMed: 24550386) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. Yes, ab24838 and ab24839 can be used together in a sandwich ELISA as they recognize different regions of the peptide epitope tag. We unfortunately do not have information regarding the antibodies' association and dissociatio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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